Regulatory

Part:BBa_K5507000:Design

Designed by: josiazhang josiazhang   Group: iGEM24_HW-ShanghaiUnited   (2024-09-29)


Ptac


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The tac promoter combines elements from both the lac and trp promoters, providing robust transcription levels. Its expression can be tightly regulated by using an inducer like IPTG, allowing precise control over gene expression timing and levels. Importantly, the tac promoter can be used in various bacterial hosts, making it suitable for different expression systems. It works well in systems that require high protein yields, often essential for research and industrial applications.


Source

Ptac from pKK223-3 plasmid.

References